ENHANCED COMPOUND-ENZYMES- -ASSISTED EXTRACTION OF POLYSACCHARIDES FROM Cornus officinalis

The process of ultrasonic-enhanced compound-enzymes-assisted extraction of polysaccharides from the fruit of Cornus officinalis was optimized by response surface methodology (RSM). The influence of three different factors on the yield of C. officinalis polysaccharides (COP) was studied. The results showed that the optimal conditions were extraction temperature 49.6 °C, ultrasound time 40.41 min, and ultrasound power 308.07 W. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and were analyzed by analysis of variance (ANOVA). The 3-D response surface plot and the contour plot derived from the mathematical models were applied to determine the optimal conditions. Under modified conditions, the experimental yield of polysaccharides was 11.02±0.41%, which was well in agreement with the value predicted by the model. A good 1,1-diphenyl-2-picryldydrazyl (DPPH) radical scavenging activity of COP extracted by ultrasonic-enhanced compound-enzymes-assisted extraction was observed.